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La Trobe University is a public research university based in Melbourne, Victoria, Australia. Its flagship campus is located in the Melbourne suburb of Bundoora. The university was established in 1964, becoming the third university in the state of Victoria and the twelfth university in Australia. La Trobe is one of the Australian verdant universities and part of the Innovative Research Universities group.

La Trobe's original and principal campus is located in the Melbourne metropolitan area, within the northern Melbourne suburb of Bundoora. It is the largest metropolitan campus in the country, occupying over 267 hectares (660 acres). It has two other major campuses located in the regional Victorian city of Bendigo and the twin border cities of Albury-Wodonga. There are two smaller regional campuses in Mildura and Shepparton and a city campus in Melbourne's CBD on Collins Street and in Sydney on Elizabeth Street.

La Trobe offers undergraduate and postgraduate courses across its two colleges of Arts, Social Science and Commerce (ASSC) and Science, Health and Engineering (SHE) .In 2015, the Times Higher Education World University Rankings ranked it in the top 100 universities under 50. In the 2021 QS World University Rankings, it was ranked in the top 300 universities for arts and humanities.